REVIEW+OF+CAFG+LAB+(Cloning+a+Fluorescent+Gene)

Here is a powerpoint presentation of all the steps of the CAFG lab. It is very brief, and the PCR movie is not in this file. The PCR movie will be uploaded soon. If there are any questions, please post comments or email us.

media type="custom" key="10758688" = = =__The 4 Major Steps__=


 * 1) **Polymerase Chain Reaction (PCR):**DNA template (which has the GFP gene) is used to amplify millions of copies of GFP
 * //Key Reagents: Taq Polymerase enzyme, primers and dNTPs//
 * 1) **Ligation:**The GFP insert is ligated to a linearized vector to create a circular plasmid
 * //Key Reagent: Ligase enzyme//
 * 1) **Transformation:**E.coli bacteria is transformed with the plasmid via heat-shocking.
 * //Key Reagent: Competent bacterial cells//
 * 1) **Plating & Selection:**The transformed bacteria (with the plasmid) are plated and selected for by using antibiotic resistance
 * //Key Reagents: LB-Agar plates with ampicillin//