Week+of+October+17

__October 17th__ Today, we did ran a gel of our digest products and a control of concentrated DNA to compare our results and see if the restriction enzymes worked properly to cut the GFP out of the plasmid.

^This is a photo (from google images) of what restriction enzymes do in cutting at only specific places based on coding.

If the gel is run properly, we would see 2 brights bands in the gel when it is photographed. One band will be the vector and the other band will be the GFP. ‍When we looked at the photograph of our results ‍, we found many more bands than expected. This means that the enzymes that were supposed to be coded for the one cutting sight each actually made many cuts instead of the two that we had anticipated. Wednesday we will have to do another digest and gel each with only one restriction enzyme in order to find out which enzyme is not cutting at the desired site so that we can find another enzyme.



This is a photograph of what generic gel results look like. The bands are the readings based on the number of base pairs of the products. In our results, we wanted only two of these bands, but found many.

(photo from google images)

__October 19th__ Because Monday's gel showed that the enzymes made too many cuts, we did a single digest using only the EcoR1 enzyme. When we run a gel of this digest next it will tell us whether the EcoR1 or the Age1 was the enzyme that had multiple cutting sites.