October+10-14

__**October 10, 2011**__ Today we nano-dropped our DNA from last week and the test results were good. We tested our two colonies we made into 4 tubes. We made more PCR using April's taq for one and the green mix for the other, 1 used my DNA colony 1A for both PCRs

For Aprils Taq For the Green Mix
 * ~ **Reagent** ||~ **Amount** ||
 * 5x PCR Buffer || 4 microliters ||
 * DNTP || 5 microliters ||
 * GFP For. || 3 microliters ||
 * GFP Rev. || 3 microliters ||
 * DNA Template || 1 microliter ||
 * Taq Polymerase || 1 microliter ||
 * Water || 3 microliters ||
 * < **Total** ||< **20 microliters** ||
 * ~ Reagent ||~ Amount ||
 * Green Mix || 10 microliters ||
 * GFP For. || 3 microliters ||
 * GFP Rev. || 3 microliters ||
 * DNA Template || 1 microliter ||
 * Water || 3 microliters ||
 * **Total** || **20 microliters** ||

October 12, 2011 We prepared another gel to test our PCR product using our DNA made from the previous week. From the gel we could tell that both Colony 1A and 1B were succesful using the green mix and April's Taq.


 * ~ Reagent ||~ Amount ||
 * Buffer || 2 microliters ||
 * Plasmid || 5 microliters ||
 * Agel || 1 microliter ||
 * ECPRl || 1 microliter ||
 * BSA || 2 microliters ||
 * Water || 9 microliters ||
 * **Total** || **20 microliters** ||