January+2012

For this month, Francis and I went over my paper from last semester and we focused in on what I will be doing for this semester. I will be working on doing Real Time PCR or Quantitative PCR. I will be comparing the number of expressed normal genes of 5 candidates to those of knockdown gene expression. I learned about the cell responsible for regeneration and what parts go along with it. I will also be comparing a normal gene expression to that of a knockdown gene expression. I also learned about how we will be testing our Real-Time PCR using a gel. We will do either mass spec or take a transmembrane receptor along with ligand.

Below is a video on Real-Time PCR that is from Applied Biosystems. I believe we will be using the SYBR green-dye process for our real-time pcr.

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And this link leads to the website which has many different links to the advantages of Real-Time PCR and how it compares to other PCRs.The benefits of Real Time PCR is that we can actually get real quantitative data that we can graph and analyze. We can compare data on the graph and compare the different genes to each other. We can get useful numbers to use in our data and find our what genes are close to our control. I read the section on Real-Time vs. Traditional and watched the video above to learn about what I will be doing in February. The tutorial video talked about what real time pcr actually is and how is is useful and what comes from it. It also went a little into how the graphs works and what different ways you can use pcr, like using SYBR green-dye for example. The real time pcr machine measure the florecence intensity and that is how we get numbers for our graphs. Overall, Real Time PCR is very beneficial.

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For purpose of confidentiality, the names of the genes, ligands, and transmembrane receptors will be kept private and be renamed as gene, ligand, or transmembrane receptor. We do know the names but the data has yet to be published.