TRANSFROMATION+Troubleshooting

=In the week of October 3-7, we did not find any problems with the bacterial cells, heat shocking or agar plates.= =Transformation with the positive control (5uL DNA template) produced several hundred GFP colonies on the arabinose-ampicillin agar plates.= =We did grow the transformed cells overnight in antibiotic-free SOCS medium before plating.= =Transformation with the ligation product produced no colonies. This is due to a problem with the ligation step.=