October+31-+November+4

October 31, 2011
Today we did a mini-prep of our 8 different culture tubes. We also decided on which primers to use for cutting our vector. We also looked at the gel from our single digest and we concluded that Ecor1 didn't cut. //**Mini-Prep **//
 * 1) Dump out the medium -- pellet in the bottom
 * 2) Add 250 microliters r3 buffer which is used to resuspend the cells
 * 3) Transfer to a 1 mililier tube
 * 4) Votrex for 30 seconds
 * 5) Add 250 microliters L7 -- invert tube 10 times
 * 6) Wait 5 minutes
 * 7) Add 350 microliters M -- invert 10 times -- this will form a percipitate
 * 8) Centrifuge for 10 minutes -- clear solution will stick to the column
 * 9) Centrifuge for 1 minute -- add 500 microliters W10 (ethonal)
 * 10) Centrifuge for 1 minute 00 200 microliters of W9 (ethonal)
 * 11) Centrifuge for 1 minute

November 2, 2011
media type="custom" key="11325736" Today we looked at our gel using Ecor1, BamH1, and Sma1 as shown above ^. And we also went through our vector map in detail: media type="custom" key="11325770"