September+5+-+9

**September 6, 2011**

Pre-Lab Questions: Set 1  1. How many microliters are there in 2.0438 milliliters? 2043.8µ  2. Should you ever use an air displacement pipette without a tip attached? Never use a pipette without a tip, you will contaminate the instrumetnt

Pre-Lab : Set 2  1. 5' TACCGATCGTTTCCT 3' 5' CTTCTTGAAAAGTGA 3' 5' CCTCAACAGGGTTAA 3' 5' GAACAACTTAATCTA 3'  2. DNA strands would you get after 35 cycles of PCR amplification after starting with one strand? 2^35 = 34,359,738,370 3. Name two possible reasons for plateauing. - the number of original primers decreases as the number of new DNA severely increases - The original strands of DNA become weaker and less susceptible to the lessing primers

Pre-Lab : Set 3  1. 3' CTACCGTATGCCTG 5'

Pre-Lab : Set 4  1. Which type of organism would be easiest to transform: bacteria, plants, mice, or humans? - Considering some bacteria are single-celled I would think that transforming bacteria would be the easiest and then plants, mice and humans