Techniques+and+Projects 

Molecular Biology

 * PCR, Real Time PCR, Touchdown PCR
 * PCR primer design
 * DNA Gel electrophoresis
 * data analysis including gels and qRT-PCR
 * nucleic acid concentration analysis with NanoDrop
 * Molecular Cloning
 * DNA digestion, ligation and gel extraction
 * bacterial culture and small scale plasmid prep
 * plasmid transformation into E. Coli
 * Proper use of lab equipment including centrifuge and incubation
 * Restriction Digest

Histology

 * tissue sectioning and placement onto slides (zebrafish eyes)
 * analysis of tissue section slides with microscopy

Bioinformatics

 * extensive understanding of ncbi including:
 * search engine
 * nucleotide database
 * BLAST
 * ENTREZ
 * literature search with PubMed
 * Utilized OMIM and UniProt
 * Gene Ontology Analysis

MATLAB

 * create a GUI
 * bioinformatic matlab programs
 * read and generate code

General Lab Techniques

 * Pipette
 * Plating
 * Centrifuge
 * Incubator