EL's+Logbook+week+of+Nov+19


 * 11/19/07**


 * ===Information=== ||
 * Nuclear pore complexes (NPCs) are anchoring sites of intranuclear filaments of 3-6 nm diameter that are coaxially arranged on the perimeter of a cylinder and project into the nuclear interior for lengths varying in different kinds of cells. Using a specific monoclonal antibody we have found that a polypeptide of approximately 190 kD on SDS-PAGE, which appears to be identical to the recently described NPC protein "nup 153," is a general constituent of these intranuclear NPC-attached filaments in different types of cells from diverse species, including amphibian oocytes where these filaments are abundant and can be relatively long. We have further observed that during mitosis this filament protein transiently disassembles, resulting in a distinct soluble molecular entity of approximately 12.5 S, and then disperses over most of the cytoplasm. Similarly, the amphibian oocyte protein appears in a soluble form of approximately 16 S during meiotic metaphase and can be immunoprecipitated from egg cytoplasmic supernatants. We conclude that this NPC protein can assemble into a filamentous form at considerable distance from the nuclear envelope and discuss possible functions of these NPC-attached filaments, from a role as guidance structure involved in nucleocytoplasmic transport to a form of excess storage of NPC proteins in oocytes.
 * Nuclear pore complexes (NPCs) are anchoring sites of intranuclear filaments of 3-6 nm diameter that are coaxially arranged on the perimeter of a cylinder and project into the nuclear interior for lengths varying in different kinds of cells. Using a specific monoclonal antibody we have found that a polypeptide of approximately 190 kD on SDS-PAGE, which appears to be identical to the recently described NPC protein "nup 153," is a general constituent of these intranuclear NPC-attached filaments in different types of cells from diverse species, including amphibian oocytes where these filaments are abundant and can be relatively long. We have further observed that during mitosis this filament protein transiently disassembles, resulting in a distinct soluble molecular entity of approximately 12.5 S, and then disperses over most of the cytoplasm. Similarly, the amphibian oocyte protein appears in a soluble form of approximately 16 S during meiotic metaphase and can be immunoprecipitated from egg cytoplasmic supernatants. We conclude that this NPC protein can assemble into a filamentous form at considerable distance from the nuclear envelope and discuss possible functions of these NPC-attached filaments, from a role as guidance structure involved in nucleocytoplasmic transport to a form of excess storage of NPC proteins in oocytes.

Niemann Pick type C (NPC) is a rare neurovisceral disorder caused by mutations in the NPC protein. This protein is located in late endosomes and is responsible for the post-lysosomal trafficking of cholesterol. NPC mutant cells are characterized by marked accumulation of unesterified cholesterol and sphingolipids in an aberrant compartment that bears both late endosomal and lysosomal markers. NPC is important not only for traffic of lipids but also of some proteins ( transferrin receptor, mannose 6-P receptor and some GPI anchored proteins).Some drugs induce the Niemann Pick phenotype in normal cells. The best characterized as acting on the NPC protein is 3-b[2diethylamino)ethoxy]androst-5-en-17-one, also known as U18666A.METHODS: Previous work in our laboratory have shown that cholesterol is required for HIV infectivity and structural integrity. Based on the role of the NPC protein in cholesterol metabolism we decided to evaluate its possible role in HIV infection.

Video Link [|Gene Trasfer and Gene Therapy] [|Biological Patter Formed: From Arrhythmias to Embryos] ||
 * ===What I did & Opinion=== ||
 * http://ihome.cuhk.edu.hk/~b400559/bioinformatics ||
 * http://ihome.cuhk.edu.hk/~b400559/bioinformatics ||


 * 11/20/07**


 * ===Information=== ||
 * * Explore NCBI websitehttp://www.ncbi.nlm.nih.gov/Search -> CoreNucleotide -> put EF619930 (this is an example)
 * * Explore NCBI websitehttp://www.ncbi.nlm.nih.gov/Search -> CoreNucleotide -> put EF619930 (this is an example)

There is a sequence of EF619930 ORIGIN 1 gaatagcggc gcgattcgcc cttagagttt gatcctggct cagattgaac gctggcggca 61 tgccttacac atgcaagtcg aacggcagca cgggtgcttg cacctggtgg cgagtggcga 121 acgggtgagt agtgcatcgg aacgtaccca gtagtggggg ataactaccc gaaagggtgg 181 ctaataccgc atatactgtg cgcaggaaag caggggaccg taaggccttg tgctactgga 241 gcggccgatg tcagattagc tagttggtgg ggtaaaggcc taccaaggcg acgacctgta 301 gcgggtctga gaggatgatc cgccacactg ggactgagac acggcccaga ctcctacggg 361 aggcagcagt ggggaatttt ggacaatggg ggcaaccctg atccagccat gccgcgtgag 421 tgaagaaggc cttcgggttg taaagctctt tcggacggaa agaaaacgga tgggttaata 481 ccctgttcgg atgacggtac cgtcataaga agcaccggct aactacgtgc cagcagccgc 541 ggtaatacgt agggtgcgag cgttaatcgg aattactggg cgtaaagcgt gcgcaggcgg 601 ttgattaaga caggcgtgaa atccccgggc ttaacctggg aactgcgctt gtgactggtc 661 ggctagagta cggcagaggg gggtggaatt ccacgtgtag cagtgaaatg cgtatagatg 721 tggaggaaca ccgatggcga atgcagcccc ctgggccgat actgacgctc atgcacgaaa 781 gcgtgggtag caaacaggat tagataccct ggtagtccac gccctaaacg atgtcaacta 841 gttgttggga gggtaaaacc ttttagtaac gtagctaacg cgtgaagttg accgcctggg 901 gagtacggcc gcaagctaaa actcatagga attgacggga cccgcacaag cggtggatga 961 tgtgattaat tcgatgcaac gcgaaaacct tacctactct tgacatgtca gagatccctg 1021 agagattggg gtgctgctgc aagaaatctg aacacagtgc tgcatgactg tcgtcagcct 1081 cgtgtccgtg agatgtagga taagatccgc aagcggtcct aaccattgtc gtaagtggcc 1141 atcaataggg ttggggccac ttttaacc

Take 3 characters to match the amino acid protein sequence. Fine the synthesis point by using anaylsis program. -> There are mostly 3 possibilities that sequence can be. ||
 * ===What I did & Opinion=== ||
 * 1. I need to learn more about sequences in order to organize analysis program. ||
 * 1. I need to learn more about sequences in order to organize analysis program. ||


 * 11/21~25/07**


 * ===Information=== ||
 * HAPPY THANGSGIVING! ||
 * ===What I did & Opinion=== ||
 * ===What I did & Opinion=== ||