Week+of+October+31

__October 31st__ Today we did mini-preps of all 8 culture tubes. Then, we looked at the vector (plasmid) map to decise which enzymes to use for cutting the plasmid- we will use Nhe1 and Sma1 to cut out the GFP. However, we will also use BamH1 and Sma1 enzymes first in order to verify the plasmid. BamH1 should give us three fragments, and Sma1 should linearize the plasmid. We also looked at the photo of the gel from the single digest.
 * Results:
 * We saw the same results as we would with undigested DNA.
 * This means that teh EcoR1 did not cut for some reason.
 * Aprell is going to rerun the single just to confirm these results.

__November 2nd__ We ran a gel containing: Results:
 * Old Peyer plasmid + EcoR1
 * pGlo + BamH1
 * pGlo + Sma1
 * The old Peyer plasmid + EcoR1 was linearized as it was supposed to be. This tells us that in the previous single digest, we must not have added enzyme to the digest.
 * pGlo + BamH1 gave two visible fragments. This is exactly the results that we would want to see, even though the BamH1 one really gives three fragments.
 * The loading buffer does not allow us to see fragments less that 500 base pairs.
 * The three fragments for BamH1 should be around >4 kp, < 1kb, and <.5 kb.
 * pGlo + Sma1 linearized the plasmid as it should.

(From Google Images)