PreLab+Questions

__PreLab Questions__ ‍‍‍‍‍‍Set 1:‍‍‍‍‍‍ 1. 2.0438mL x (1000 microliters/1mL) = 2043.8 microliters 2. You should never touch the tip of an air displacement pipette to a liquid without a tip being attached because this will contaminate the instrument.

Set 2: 1.‍‍‍‍‍‍ 5'_ATGGCTAGCAAAGGA_3' 5’_ATTTGTAGAGCTCAT_3’ 2. 2^35 = 3.35973837x10^10 strands of DNA ‍‍‍‍‍‍‍3.‍‍‍‍‍‍‍ The replication begins to plateau because the number of primers available to attach to the template begins to decrease over time, and eventually the taq loses its activity causing less primers to bond to the DNA template.

Set 3: 1. ‍‍‍‍‍‍5'_GATGGCATACGGACA_3' 3'_ACTACCGTATGCCTA_5' 2. 3. 5'_GATCATATGAACGAATT_3' and 5'_AATTCACAATCTGCCCTTTC_3' after the EcoRl cuts after the AATT. When the DNA ligase is added these two strands will reform a circle with base pairs as follows.