RM's+Week+of+July+14

Back to RM's Summer Research


 * July 14, 2008**

Made a new stock solution. Solution will be annealed overnight.
 * 80 ul of DNA (5 ul of each strand)
 * 20 ul 10x Mg 2+/TAE buffer
 * 100 ul 18 mega ohm H20


 * July 15, 2008**

Made a new sample with just 10 ul of the stock solution, and without further dilution. It worked for AL yesterday, so perhaps there were some contaminants in previous stock solutions that prevented me from using just the stock solution on my samples. I went to the AFM and captured images. I had to restart the machine, however, because I wasn't obtaining clear images, and thought that rebooting the system may clear the problem. It did. This sample did not yield as many interesting images as my sample from 7/10/08 did. I was in the AFM for about 3 hours.


 * July 16, 208**

I was in the AFM, viewing previous samples. I tried obtaining images from the sample made yesterday.


 * July 17, 2008**

I was in the AFM, again. However, before going in, I made a new sample with 2 ul of the DNA solution and 8 ul of the buffer. It was still quite concentrated when I viewed it under the micrscope. I also washed the sample made on 7/10/08 with 18 mega ohm H20. Yesterday, it was very contaminated. Today, after rinsing it, it was much cleaner under the microscope. Rinsing worked well because many of the contaminants on the surface are water soluble. The nitrogen tank was also replaced today.


 * July 18, 2008**

I viewed the new sample prepared yesterday.