Week+of+September+26

__September 26th__ Troubleshooting: Last week, the found that the PCR didn't work. Today, we tested the template and the primers to see if they were right to do PCR. To test these reagents, we did a Nanodrop of each. The Nanodrop tells the concentration each. Results: There is no DNA in the DNA template that we used. The primers have about 6‍‍.0 concentration ‍‍. (Not great but it is enough for PCR)

Starting over: We found DNA from an older experiment that has a high enough concentration for PCR and made new PCR products following the same process as before. Wednesday, we will run another gel to see if the PCR worked this time.

__September 28th__ When redoing the PCR on Monday, we did three different combinations: one with the green mix taq, one with Aprell's taq from her lab, and another with a third type of taq ploymerase. Today we ran a new gel of these "PCR products" to see if the PCR worked. After running the gel, we found the only one that did not produce PCR products was the one using Aprell's taq. Therefore we were able to find the polymerase that would produce the results we wanted.