Digest

__Digest Procedure:__
 * 1) Add 2 microliters buffer.
 * 2) Add 5 microliters Plasmid
 * 3) Add 2 microliters BSA
 * 4) Add 9 microliters water
 * 5) If single digest add 2 microliters of the single restriction enzyme. **Or** If double digest add 1 microliter of each restriction enzyme.
 * 6) Place tube in a water bath or incubator and let sit overnight to guarentee pure GFP.