Week+of+Sept+5+-+9

September 6, 2011  __‍Pre-Lab Questions: ‍Set 1 ‍__ ‍ 1. How many microliters are there in 2.0438 milliliters? 2043.8µ 2. Should you ever use an air displacement pipette without a tip attached? Never use a pipette without a tip, you will contaminate the instrument

__Pre-Lab : Set 2__ 1‍. 5' TACCGATCGTTTCCT 3' 5' CTTCTTGAAAAGTGA 3' 5' CCTCAACAGGGTTAA 3' 5' GAACAACTTAATCTA 3' ‍

2. DNA strands would you get after 35 cycles of PCR amplification after starting with one strand? 2^35 = 34,359,738,370 3. N‍ame two possible reasons for plateauing. ‍ - the number of original primers decreases as the number of new DNA severely increases - The original strands of DNA become weaker and less susceptible to the lessing primers

__Pre-Lab : Set 3__ 1. ‍3' CTACCGTATGCC‍TG 5' ‍ ‍

__Pre-Lab : Set 4__ 1. Which type of organism would be easiest to transform: bacteria, plants, mice, or humans? - Considering some bacteria are single-celled I ‍would think that transforming bacteria would be the easiest ‍and then plants, mice and humans