EL's+Logbook+Week+of+Dec+3


 * 12/03/07**

Zebrafish chromatophores, shown here mediating background adaptation, are studied by scientists A Zebrafish Pigment Mutant. The mutant called bleached blond was produced by insertional mutagenesis. The embryos in the picture are four days old. At the top is a wild-type embryo, below is the mutant. The mutant lacks black pigment in the melanocytes because it fails to synthesise melanin properly.Zebrafish embryonic development provides advantages over other vertebrate model organisms. Although the overall generation time of zebrafish is comparable to that of mice, zebrafish embryos develop rapidly, progressing from eggs to larvae in under three days. The embryos are large, robust, and transparent and develop externally to the mother, characteristics which all facilitate experimental manipulation and observation. [|[5]] Their nearly constant size during early development facilitates simple staining techniques, and drugs may be administered by adding directly to the tank. Unfertilised eggs can be made to divide, and the two-celled embryo fused into a single cell, creating a fully homozygous embryo. A common reverse genetics technique is to reduce gene expression or modify splicing in zebrafish using Morpholino antisense technology. Morpholino oligonucleotides are stable, synthetic macromolecules that contain the same bases as DNA or RNA; by binding to complementary RNA sequences, they reduce the expression of specific genes. The journal Genesis devoted an issue to research using Morpholino oligos, mostly in //D. rerio//. Morpholino oligonucleotides can be injected into one cell of a zebrafish embryo after the 32-cell stage, producing an organism in which gene expression is reduced in only the cells descended from the injected cell. However, cells in the early embryo (<32 cells) are interpermeable to large molecules, allowing diffusion of Morpholinos between cells. A known problem with gene knockdowns in zebrafish is that, because the genome underwent a duplication after the divergence of ray-finned fishes and lobe-finned fishes, it is not always easy to silence the activity one of the two gene paralogs reliably due to complementation by the other paralog. || I want to investigate further after finishing my project! ||
 * ===Information=== ||
 * =A Quick Glance to Zebrafish= Why do I research about zebrafish?because the vertebrae of zebrafish is close to the structure of humans....When I analysis the sequence of DNA in the vertebrae,I will figure out interesting sequence. ( You can take a guess, of course )However, I will not actually deal with zebrafish although I can relate bioinformatics information to zebrafish project.
 * =A Quick Glance to Zebrafish= Why do I research about zebrafish?because the vertebrae of zebrafish is close to the structure of humans....When I analysis the sequence of DNA in the vertebrae,I will figure out interesting sequence. ( You can take a guess, of course )However, I will not actually deal with zebrafish although I can relate bioinformatics information to zebrafish project.
 * ===What I did & Opinion=== ||
 * 1. I searched some information about zebrafish for fun.
 * 1. I searched some information about zebrafish for fun.


 * 12/04/07**
 * ===Information=== ||
 * ===What I did & Opinion=== ||
 * Today, I printed an e-mail from Dr. Loughran about milestone. I will work on milestone from today because I actually do not have enough time to work on milestone on next week or so. I have sent and will send an e-mail to Jill to discuss about milestone. Also, I will organze what we have done for this project. ||
 * ===What I did & Opinion=== ||
 * Today, I printed an e-mail from Dr. Loughran about milestone. I will work on milestone from today because I actually do not have enough time to work on milestone on next week or so. I have sent and will send an e-mail to Jill to discuss about milestone. Also, I will organze what we have done for this project. ||
 * Today, I printed an e-mail from Dr. Loughran about milestone. I will work on milestone from today because I actually do not have enough time to work on milestone on next week or so. I have sent and will send an e-mail to Jill to discuss about milestone. Also, I will organze what we have done for this project. ||

2. I copied every record of my logbook from the very first week to organize the milestone. Actually, some data are dupilicated, so I need to get rid of them, in case of unneccessity. ||
 * 12/05/07**
 * ===Information=== ||
 * ===What I did & Opinion=== ||
 * 1. I will make an outline for milestone on next Tuesday (on which is my next meeting) with Jill.
 * ===What I did & Opinion=== ||
 * 1. I will make an outline for milestone on next Tuesday (on which is my next meeting) with Jill.
 * 1. I will make an outline for milestone on next Tuesday (on which is my next meeting) with Jill.


 * 12/06/07**
 * ===Information=== ||
 * ===What I did & Opinion=== ||
 * 1. I still take the time to organize my log book. It is not that stuructural, so more time is required... ||
 * ===What I did & Opinion=== ||
 * 1. I still take the time to organize my log book. It is not that stuructural, so more time is required... ||
 * 1. I still take the time to organize my log book. It is not that stuructural, so more time is required... ||

Well, even though I can make a simple outline for this milestone, but I want to discuss about this milestone with Jill so that I can get more detailed information. I am going to make a milestone by using photoshop! I will upload both hand-written and computer graphic version of milestone on this weekend and next week. ||
 * 12/07/07**
 * ===Information=== ||
 * ===What I did & Opinion=== ||
 * Dr. Loughran asked us to make milestone map by today because he will be out of town on mid of next week.
 * ===What I did & Opinion=== ||
 * Dr. Loughran asked us to make milestone map by today because he will be out of town on mid of next week.
 * Dr. Loughran asked us to make milestone map by today because he will be out of town on mid of next week.