September+12-16

Today we met with April and Francis at Jordan and discussed our lab. We went over the steps of the GFP lab and we will be using Jelly Fish DNA for our lab. __PCR: Polymerase Chain Reaction__ 1. The first step in PCR is the breaking of the H+ bonds of dsDNA which occurs at about 95 C 2. Anneling of Primers is second. A primer is a short strand of single DNA designed to bind ends of DNA of interest. - There are forward and reverse primers going from 5' to 3' - it occurs between 50 - 60 C 3. Extentions (Elongation) of DNA - 70 C We also learned about dNTP - free floating nucleotides DNA template = parent strand Taq Polymerase: thermal stable polymerase that withstand high temperatures for our PCR __Ligation__ The second step in our GFP cloning is ligation which is taking linear GFP and adding a cut vector to create a plasmid with GFP DNA inserted - --GFP-- + cut vector = GFP plasmid __Transformation__ - insert plasmid into bacteria - heat shock __Plating and Selection__ Take a colony off the plate and see if it contains our GFP DNA We also went over the difference between blunt and sticky end enzymes. A blunt enzyme is when the nucleotides are cut even and sticky ends are when they are cut unevenly so there are loose ends for other dNTP's to attach too. Today was mostly review of the lab and explanations in what we will be doing for next week. Today we met and worked on using microsoft excel and it's different mathmatical and programing features. Below is an attachment of the work we did in excel for today. We also finished installing the MATLAB data to use for next week and recieved a packet in which we will be using for the program. We were asked to read Chapter 1 for next Friday.
 * September 14, 2011**
 * September 16, 2011**

[|Paige Dausinas.xlsx]