October+17-20

October 17, 2011
Today we ran a double digest and by looking at the gel we ran afterward we discovered that it had failed. We are now looking to see why it cut in too many places on our vector.

October 19, 2011
Today we did a Ecor1 digest and used and made the master mix for it including the following: (microliters) ||
 * ~ 6x(microliters) ||~ Reagent ||~ 1x
 * 12 || React 3 Buffer ||  ||
 * 12 || 10x BSA ||  ||
 * .3 || Enzyme ||  ||
 * 83.7 || Water (H2O) ||  ||
 * || DNA || 2 ||

We then used 18 microliters of the master mix and 2 microliters of DNA for each tube.